Seminar: Vật liệu Nano cho ứng dụng điều trị ung thư và cảm biến chọn lọc phân tử sinh học

4/3/2019 1:18 PM

Thời gian: 10:00 - 11:30 ngày 5/4/2019

I. Địa điểm: Phòng họp khoa Dược, 112-113 nhà C1, Trường Đại học PHENIKAA, Yên Nghĩa, Hà Đông, Hà Nội

II. Thành phần:

     1. Phía Hàn Quốc

Prof. Yong-Ill Lee, Department of Chemistry, Changwon National University, Korea

Dr. Bui The Huy, Department of Chemistry, Changwon National University, Korea

Dr. Nikolai Tsvetkov, Graduate School of Energy, Environment, Water and Sustainability, Korea Advanced Institute of Science and Technology Republic of Korea

      2. Phía Trường Đại học PHENIKAA

PGS. TS. Pham Thành Huy, Hiệu trưởng Trường Đại học PHENIKAA

GS.TS. Nguyễn Văn Hiếu, Phó hiệu trưởng Trường Đại học PHENIKAA

Cùng các nhà khoa học, giảng viên, cán bộ nghiên cứu Trường Đại học PHENIKAA

III. Nội dung:

Chủ tọa: GS. TS. Nguyễn Văn Hiếu

Chương trình chi tiết:

Speaker

Title

Time

Prof. Yong-Ill Lee

Targeted Imaging for Cancer Cells and Selective Opt-sensing of Biochemical Molecules Using Novel Nanomaterials

10:00-10:30

Dr. Nikolai Tsvetkov

Control of the chemical composition and electronic structure at surfaces and interfaces for efficient charge and ion transfer

10:30-11:00

 

Prof. Nguyen Van Hieu and all

 

 

Discussion

 

11:00-11:30


Prof. YONG-ILL LEE

Department of Chemistry, Changwon National University, Korea

Adress: Anastro Laboratory, Department of Chemistry

Changwon National University

Changwon 641-773, KOREA

Fax: +82-55-213-3439

Tel: +82-55-213-3436

Email: yilee@changwon.ac.kr

Biographical Sketch

- Yong-Ill Lee is a Professor in the Department of Chemistry, Director of the BK21 Plus Nanobio Research Center at Changwon National University (CWNU), Changwon, Korea.

- He received his M.Sc. in Polymer Science in 1991 and a Ph.D. in Analytical Chemistry in 1993 from the University of Massachusetts (UMASS), USA under the guidance of Prof. Joseph Sneddon. His dissertation was directed for the development of laser-induced breakdown spectrometry (LIBS) and its application for solid sample analysis. He received several awards including Outstanding Graduate Student Award and Professional Development Award during his stay in UMASS. After graduation, He was an assistant professor at Konyang University, Nonsan, Korea until 1997, when he joined the faculty of the Department of Chemistry at Changwon National University. He had been a Head of the Chemistry Department from 1998 to 2000 and served as the Directors of Research Institute of Basic Sciences and the Central Institute of Instrumental Facilities from 2003 to 2004 at CWNU. He had also been a Vice President of CWNU from 2007 to 2008.

- His research interests include analytical spectroscopy in general, laser spectroscopy (time and space-resolved), more specifically the development and application of new plasma techniques for advanced materials, fluorescence spectrometry for biomolecules, gas-phase reaction and kinetic method for chiral analysis by mass spectrometry, photoluminescence of lanthanide composite materials, micro- and nanostructures of materials and optosensing biochemical molecules. He is an author of over 300 scientific publications, 10 patents, two books on laser-induced breakdown spectrometry and 9 book chapters on laser-based analytical spectrometry.

- He received the 1999 Meritorious Service Award and the 2004 Outstanding Research Professor Award from CWNU, the 2003 Outstanding Scholar Award from the Korean Chemical Society (Analytical Chemistry Division), the 2007 Sunil Scientific Award from the Korean Society for Analytical Sciences, the 2009 A.A. Benedetti-Pichler Award from the American Microchemical Society. As a result of his efforts, he is performed several research projects as a Principal Investigator from the Korean government agency, Korea Research Foundation (KRF). Recently, His research group has been selected in BK21+ program for nanobioscience research supported by Korean Ministry of Education. He is currently an editor for Asia of Applied Spectroscopy Reviews, editorial board member of the Microchemical JournalSpectroscopy Letters and also an Advisory Board of Mass Spectrometry Letters.

 

Targeted Imaging for Cancer Cells and Selective Optosensing of Biochemical Molecules Using Novel Nanomaterials

 Yong-Ill Lee*

Department of Chemistry, Changwon National University

Changwon 51140, The Republic of Korea, E-mail: yilee@changwon.ac.kr

Nanomaterials are well known to possess excellent electrical, optical, thermal, catalytic properties and strong mechanical strength, which offer great opportunities to construct nanomaterials-based sensors or devices for determining various kinds of biochemical molecules in water and biological sample matrices. Various nanomaterials including upconversion nanoparticles, gold nanoparticles, carbon nitrides and quantum dots have been extensively investigated in my laboratory for highly selective/sensitive sensing toxic metal ions, toxic gases, drugs and biochemical molecules with simplicity. Novel multiple emitting amphiphilic conjugated polythiophene-coated CdTe quantum dots for picogram level determination of the 2,4,6-trinitrophenol (TNP) explosive are developed. Portable, cost effective, and simple to use paper strips and chitosan film are successfully applied to visually detect as little as 2.29 pg of TNP. The possibility of utilizing a smartphone with a color-scanning APP in the determination of TNP is also established. Novel “turn off-on” optosensors based on amphiphilic thiophene copolymers coated CdTe quantum dots were also developed for the dual detection of heparin and spermine. The emission of the nanoparticles is found to be quenched in the presence of heparin by electron transfer mechanism through electrostatic and hydrogen bonding interactions. We report the effective synthesis of biocompatible upconversion nanoparticles (UCNP)-loaded phosphate micelles and successful delivery of UCNPs and drugs to prostate cancer cells via secreted phospholipase A2 (sPLA-2) enzyme cleavage of the loaded micelles for the first time. The selective release of UCNPs to prostate cancer cells rather than other cells, specifically cervical cancer cells, was observed and confirmed by a range of bioimaging studies. In addition, the proposed method was successfully utilized for selective drug delivery of estramustine phosphate, anti-prostate cancer drug, to prostate cancer cells. Drug encapsulation efficiency (EE%) was found to be 81.432% and release efficiency (RE%) was found to be 91%. Encapsulated drug was tested on in vitro application, and cytotoxicity test confirmed the selective delivery of estramustine on prostate cancer cells

References

1. S.M. Tawfik, M. Sharipov, S. Kakhkhorov, M.R. Elmasry, Y.I. Lee, Advanced Science 1801467 (2019)

2. N.N. Nghia, B.T. Huy, Y.I. Lee, Microchimica Acta 186: 36 (2019)

3. S.M. Tawfik, J. Shim, D. Biechele-Speziale, M. Sharipov, Y.I. Lee, Sensors and Actuator B: Chemical 257, 734-744 (2018)

4. S.M. Tawfik, B.T. Huy, M. Sharipov, A. Abd-Elaal, Y.I. Lee, Sensors and Actuator B: Chemical 256, 243-250 (2018)

5. M. Sharipov, S.M. Tawfik, Z. Gerelkhuu, B.T. Huy, Y.I. Lee, Scientific Reports 7, 16073 (2017)

Dr. Nikolai Tsvetkov,

Graduate School of Energy, Environment, Water and Sustainability

Korea Advanced Institute of Science and Technology Republic of Korea


Biography

- Nikolai Tsvetkov is research assistant professor in Korean Advanced Institute of Science and Technology (KAIST). He got MS degree at Moscow Institute of Electronic and Mathematics in 2006 and PhD degree in Materials Science Department at KAIST in 2012. At that time he studied fundamental charge transfer processes on nano-sized interfaces for dye-sensitized solar cells and CIGS solar cells. After graduation and till 2016 he worked in MIT in the laboratory for Electrochemical Interfaces performing research on Solid Oxide Fuel Cells. In 2013 he got the best poster award in Solid State Ionics-19 meeting in Okinawa, Japan, and also an award from MIT postdoctoral association. In 2018 he won the American Ceramic Society Ross Coffin Purdy Award.

- Dr. Tsvetkov main research interests and activities are concentrated on the electronic structure and charge transfer process at surfaces and interfaces for various energy technologies including solar cells, batteries, fuel cells, and water splitting reactors. In his works he has shown how the controllable modification of the interface structure can results in drastic enhancement in the device efficiency. He is co-author of more than twenty papers in international peer-reviewed journals including Energy & Environmental Science, ACS Nano, and Nature Materials.

 

Control of the chemical composition and electronic structure at surfaces and interfaces for efficient charge and ion transfer

Nikolai Tsvetkov

Research Assistant Professor

Graduate School of Energy, Environment, Water and Sustainability,

Korea Advanced Institute of Science and Technology

Republic of Korea

Last decade, hybrid organic/inorganic and all-inorganic hetero-structures attracted increased interest due to the distinctly properties that can arise from their interfaces, such as a high optical absorption, superconductivity, high catalytic activity, or unusual magnetic behavior. Defect engineering at the surfaces and interfaces is coming into the spotlight as novel approach for the fabrication of systems with advanced properties. I will present our recent studies on defect engineering addressed to the development efficient energy conversion technologies. On the first, I will present the investigation of hybrid organic/inorganic light absorber material, methylammonium lead iodide (MAPbI), when it is in contact with different, organic or inorganic, charge transfer layers for solar cells applications. The performance of perovskite solar cells based on MAPbI3­ is limited by surface recombination at Electron Transfer Layer (ETL)/MAPbIinterface. I will show that capping a TiO2 ETL surface with Pb atoms provides a new solution to this challenge. The X-ray photoelectron spectroscopy (XPS) measurements of Pb-capped ETL surfaces showed that the oxidation state of Pb is bivalent (2+) up to a complete monolayer limit, while an excess of PbO coverage in a multilayer and/or nanocluster morphology leads to the formation of deep defect states due to the partial oxidation of Pb to the trivalent (3+) state. Also, the TRPL measurements for the perovskite/ETL interfaces revealed an approximately two-fold higher charge injection rate from the perovskite to the monolayer PbO-modified ETL compared to the pristine perovskite/ETL interface. Density functional theory calculations and experiments identify that the PbO monolayer converts the TiO2/perovskite interface into a metallic state, providing atomistic origins for about two-fold increase in charge transfer and enhanced photovoltaic efficiency from 17.8% up to 20.4%. On the other hand, the subsequent excessive Pb capping was found to create a semiconducting layer with a mismatched band alignment, explaining the increased interfacial resistance and the decreased PSC conversion efficiency observed in experiments.

On the second theme, I will present recent results on the investigation of the degradation of catalyst materials for Solid Oxide Fuel Cells. We investigated the surface of the model perovskite oxide catalyst, La0.2Sr0.8CoO3, to determine the factors behind degradation of its catalytic activity toward oxygen reduction reaction. Previously it was shown that the elastic and electrostatic interactions of the dopants, i.e. Sr, within the lattice are key drivers to the detrimental segregation and phase separation of dopants at the surface. Based on this approach, I will show how we can tune the surface charge on perovskite oxides with heterogeneous doping, and improve the chemical and electrochemical stability of the surfaces. Towards this goal, we used a novel combination of in-situ ambient pressure energy X-ray photoelectron and absorption spectroscopy. We found that the doping with cations which are less reducible that Co on B-site of perovskite, such as Ti, Zr or Hf, significantly improves stability, with up to 30x acceleration of the oxygen exchange kinetics after 54 hours in air at 550 oC achieved by Hf addition onto LSC.

Finally, I will show that the surface engineering approach can be also successfully utilized for improvement of the LiFePO4(LPF) cathode materials for Li ion battery applications. Surface chemistry modification of LPF nanoparticles with various transition metals allows to significantly improving rate performance of cathodes as well as its specific capacitance.